https://doi.org/10.15255/KUI.2017.017
Published: Kem. Ind. 66 (7-8) (2017) 373−380
Paper reference number: KUI-17/2017
Paper type: Preliminary communication
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Immobilization of Tyrosinase on (3-Aminopropyl)triethoxysilane-Functionalized Carbon Felt-Based Flow-Through Detectors for Electrochemical Detection of Phenolic Compounds

Z. Zhou, Y. Wang, Z. Q. Zhang, Y. Zhang, Y. Hasebe, Y. M. Song and C. P. Wang

Abstract

Tyrosinase (TYR) was covalently immobilized onto amino-functionalized carbon felt (CF) surface via glutaraldehyde (GA). Prior to the TYR-immobilization, primary amino group was introduced to the CF surface by treatment with 3-aminopropyltriethoxysilane (APTES). The resulting TYR-immobilized CF was used as a working electrode unit of an electrochemical flow-through detector for mono- and di-phenolic compounds (i.e., catechol, p-cresol, phenol and p-chlorophenol). Additionally, flow injection peaks based on electroreduction of the enzymatically produced o-quinone species were detected at −0.05 V vs. Ag/AgCl. The resulting TYR/GA/APTES/CF biosensor responded well to all compounds tested with limits of detection range from 7.5 to 35 nmol l^–1 (based on three times S/N ratio). Moreover, such modified electrode exhibits good stability and reproducibility for catechol. No serious degradation of the peak current was found over 30 consecutive injections.

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Keywords

tyrosinase, carbon felt, (3-aminopropyl)triethoxysilane, flow-through detector